Universiti Sains Malaysia (USM) has recognized sustainability integration as the main pathway for its transformation. This research prioritizes on the level of sustainable development (SD) and education for sustainable development (ESD) that are thematically bound but different. Centre for Global Sustainable Studies (CGSS) has been developing ‘Sustainability Assessment Methodology’ (SAM). It is used as sustainable index to assess the sustainability content of existing academic, research activities and community engagement in USM. This audit involved 43 PTJs consisting of 26 schools and 17 Centers of Excellence. The result showed 83% of academic, research activities and community engagement in USM is sustain. This index will envisage as reference for local Higher Education Institute (HEIs) in Malaysia. A comprehensive system should be developed to inculcate fast and accurate result for the entire HEIs.